汉防己甲素及其联合氟康唑对白念珠菌细胞周期影响的初步研究

中国真菌学杂志　 2014, Vol. 9 　Issue (4): 215-217.

汉防己甲素及其联合氟康唑对白念珠菌细胞周期影响的初步研究

吕霞琳, 张宏, 宋延君, 李水秀

暨南大学附属第一医院暨南大学真菌病研究所, 广州 510632

收稿日期:2014-07-03 出版日期:2014-08-28 发布日期:2014-08-28
通讯作者: 张宏，E-mail：tzhangh@jnu.edu.cn E-mail:tzhangh@jnu.edu.cn
作者简介:吕霞琳，女（汉族），硕士，住院医师.E-mail：597249066@qq.com
基金资助:
国家自然科学基金（30972660，81171542）

A preliminary research of tetrandrine and fluconazole affecting the cell cycle in Candida albicans

LV Xia-lin, ZHANG Hong, SONG Yan-jun, LI Shui-xiu

The First Affiliated Hospital, Institute of Mycology, Jinan University, Guangzhou 510632, China

Received:2014-07-03 Online:2014-08-28 Published:2014-08-28

摘要/Abstract

摘要： 目的探讨汉防己甲素联合氟康唑对白念珠菌细胞周期的影响。方法将白念珠菌CA-1菌悬液与汉防己甲素和（或）氟康唑共培养12 h，应用流式细胞仪测定空白对照组、汉防己甲素组、氟康唑组及汉防己甲素联合氟康唑组DNA含量。比较细胞周期各期DNA含量变化并计算增殖抑制率PI%，分析汉防己甲素及其联合氟康唑对白念珠菌细胞周期的影响。结果汉防己甲素、氟康唑组与对照组S期DNA含量相比，分别能增加17.25% （P=0.018）与6.54% （P>0.05），其联合运用效果更明显，S期DNA含量可增加31.52% （P=0.002）。这说明汉防己甲素能将白念珠菌细胞阻滞在S期。结论汉防己甲素能抑制白念珠菌细胞DNA合成，阻断白念珠菌细胞周期进程，抑制细胞分裂，其与氟康唑联用时阻滞作用更显著。

关键词: 汉防己甲素, 氟康唑, 白念珠菌, 流式细胞术

Abstract: Objective To explore the effect of TET (tetrandrine) and fluconazole on the cell cycle in Candida albicans.Methods Flow Cytometry was used to measure the DNA content of each cell cycle phase of the FLC (Fluconazole) sensitive strain CA-1, which was exposed to TET, FLC and TET+FLC.The effect of TET (tetrandrine) and fluconazole on the cell cycle in Candida albicans was analysed by comparing DNA content of different cell cycle phases and calculating the PI% (proliferation inhibition rate).Results Compared with control group,TET and FLC could increase the DNA content of cell cycle in S-phase by 17.25% (P=0.018) and 6.54% (P>0.05) respectively,while the combination use of TET and FLC could markedly increase S-phase DNA content by 31.52% (P=0.002).It showed that TET can arrest C.albicans cells in S phase.Conclusions TET has potent effect of against Candida albicans by means of inhibiting DNA synthesis,arrest the cell cycle and inhibiting cell proliferation,while it would be more notable when combined with FLC.

Key words: tetrandrine, fluconazole, Candida albicans, flow cytometry

中图分类号:

R379.4

吕霞琳, 张宏, 宋延君, 李水秀. 汉防己甲素及其联合氟康唑对白念珠菌细胞周期影响的初步研究[J]. 中国真菌学杂志, 2014, 9(4): 215-217.

LV Xia-lin, ZHANG Hong, SONG Yan-jun, LI Shui-xiu. A preliminary research of tetrandrine and fluconazole affecting the cell cycle in Candida albicans[J]. Chinese Journal of Mycology, 2014, 9(4): 215-217.

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