两株西沙群岛野生诺尼种子内生酵母CICC32991与CICC32993的多相分类鉴定

中国真菌学杂志　 2014, Vol. 9 　Issue (4): 199-202.

两株西沙群岛野生诺尼种子内生酵母CICC32991与CICC32993的多相分类鉴定

白飞荣¹, 刘洋¹, 姚粟¹, 李辉¹, 谭望桥², 程池¹

1. 中国食品发酵工业研究院中国工业微生物菌种保藏管理中心, 北京 100015;
2. 海南诺尼生物工程开发有限公司, 海口 570125

收稿日期:2014-03-19 出版日期:2014-08-28 发布日期:2014-08-28
通讯作者: 程池，E-mail：cheng100027@163.com；刘洋，E-mail：ly81150@163.com E-mail:cheng100027@163.com;ly81150@163.com
作者简介:白飞荣，男（汉族），硕士，工程师.E-mail：aa330768860@126.com
基金资助:
国家自然科学基金（31300008），中国食品发酵工业研究院科技发展基金（博士基金）（2012KJFZ-BS-01），北京市科技新星计划（Z141105001814095），国家微生物资源平台专项（NIMR2014-4）

Identification on two yeasts CICC32991 and CICC32993 isolated from Paracel Islands wild Noni (Morinda citrifolia L.) Seeds

BAI Fei-rong¹, LIU Yang¹, YAO Su¹, LI Hui¹, TAN Wang-qiao², CHENG Chi¹

1. China Center of Industrial Culture Collection, China National Research Institute of Food and Fermentation Industries, Beijing 100015, China;
2. Hainan Noni Biological Engineering Development Co., Ltd., Haikou 570125, China

Received:2014-03-19 Online:2014-08-28 Published:2014-08-28

摘要/Abstract

摘要： 目的通过多相鉴定方法对分离自我国西沙群岛野生诺尼种子内的两株酵母（CICC32991，CICC32993）进行准确鉴定。方法包括形态学观察试验、生理生化特征试验、以及核糖体DNA序列系统发育学分析等技术。结果形态学和生理生化试验结果显示CICC32991为Eremothecium sp.，CICC32993为Kodamaea ohmeri;系统发育分析显示CICC32991为Eremothecium coryli，CICC32993为Kodamaea ohmeri。结论综合各试验结果表明菌株CICC32991为Eremothecium coryli；CICC32993为奥默柯达菌（Kodamaea ohmeri）。

关键词: 西沙群岛, 诺尼, 多相鉴定, CICC32991, CICC32993

Abstract: Objective To distinguish the two yeasts (CICC32991,CICC32993) isolated from Paracel Islands wild Noni (Morinda citrifolia L.) seeds by the polyphasic taxonomy methods.Methods This study included morphological, physiological and biochemical characteristics of the test, as well as ribosomal DNA sequence phylogenetic analysis technology.Results Morphological and physiological test results showed the strain CICC32991 as Eremothecium sp. and CICC32993 as Kodamaea ohmeri.Phylogenetic analysis results showed CICC32991 as Eremothecium coryli and CICC32993 as Kodamaea ohmeri.Conclusion The results certified the strain CICC32991 as Eremothecium coryli and the strain CICC32993 as Kodamaea ohmeri.

Key words: Paracel Islands, Noni, Polyphasic identification, CICC32991, CICC32993

中图分类号:

Q939.5

白飞荣, 刘洋, 姚粟, 李辉, 谭望桥, 程池. 两株西沙群岛野生诺尼种子内生酵母CICC32991与CICC32993的多相分类鉴定[J]. 中国真菌学杂志, 2014, 9(4): 199-202.

BAI Fei-rong, LIU Yang, YAO Su, LI Hui, TAN Wang-qiao, CHENG Chi. Identification on two yeasts CICC32991 and CICC32993 isolated from Paracel Islands wild Noni (Morinda citrifolia L.) Seeds[J]. Chinese Journal of Mycology, 2014, 9(4): 199-202.

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