美浮特®皮肤抗菌液对致足癣真菌抗真菌后效应的体外研究

中国真菌学杂志　 2014, Vol. 9 　Issue (2): 88-92.

美浮特^®皮肤抗菌液对致足癣真菌抗真菌后效应的体外研究

史冬梅^1,2, 顾克斌³, 沈永年¹, 吕桂霞¹, 刘维达¹

1. 中国医学科学院皮肤病医院, 南京 210042;
2. 济宁市第一人民医院皮肤科, 济宁 272001;
3. 济宁市第一人民医院药剂科, 济宁 272001

收稿日期:2014-04-08 出版日期:2014-04-28 发布日期:2014-04-28
通讯作者: 刘维达，liumyco@hotmail.com E-mail:liumyco@hotmail.com
作者简介:史冬梅，女（汉族），博士，副主任医师.E-mail：shidongmei28@163.com；顾克斌，男（汉族），学士，副主任药师.E-mail：262150898@qq.com
基金资助:
国家重大科技专项资助（2012ZX09301002005001005）

Post-antifungal effect of Meifute antifungal solution against fungal induced tinea pedis in vitro

SHI Dong-mei^1,2, GU Ke-bing³, SHEN Yong-nian¹, LÜ Gui-xia¹, LIU Wei-da¹

1. Department of Mycology, Institute of Dermatology, Chinese Academy of Medical Sciences & Peking Union Medical College, Nanjing 210042, China;
2. Department of Dermatology, Jining First People's Hospital, Jining 272001, China;
3. Dermatology of Pharmacy, Jining First People's Hospital, Jining 272001, China

Received:2014-04-08 Online:2014-04-28 Published:2014-04-28

摘要/Abstract

摘要：

目的评价美浮特^®皮肤抗菌液对致足癣真菌皮肤癣菌和白念珠菌的体外抗真菌活性及抗真菌后效应。方法采用美国CLSI M27-A3和M38-A2方案测定美浮特皮肤抗菌液对足癣常见致病真菌的最低抑菌浓度（MIC）；并以白念珠菌（ATCC90028）为指示菌测定美浮特皮肤抗菌液测定时间-杀菌曲线，同时测定其对白念珠菌的抗真菌后效应（post-antifungal effect，PAFE）。结果美浮特皮肤抗菌液对4属6种57株MIC的范围为1：40~1：160、MIC₅₀为1：80、MIC₉₀为1：40；对白念珠菌的MIC范围为1：40~1：80、对皮肤癣菌的MIC范围为1：40~1：160。该抗菌液具有很强的杀菌作用，且随着药物浓度的降低，杀菌速度和程度随之变化。该抗菌液对白念珠菌0.5MIC、MIC、2MIC的PAFE分别为0.85 h、2.1 h、3.59 h；且PAFE时间的延长与药物浓度呈正相关。结论美浮特^®皮肤抗菌液对致病真菌皮肤癣菌、白念珠菌具有快速、有效、持续的杀菌作用，该抗菌液对皮肤癣菌较白念珠菌具有更强的抗真菌作用。且该抗菌液对白念珠菌具有较长时间的后效应，可以广泛应用于临床治疗皮肤癣菌及白念珠菌所致的感染。

关键词: 最小抑菌浓度, 时间-杀菌曲线, 抗真菌后效应

Abstract:

Objective To investigate antifugal activity and the post-antifungal effect (PAFE) of Meifute antifungal solution against fungal induced tinea pedis.Methods We detected the minimum inhibitory concentration (MIC) of Meifute antifungal solution against fungal common induced tinea pedis using the United States CLSI M27-A3 and M38-2 scheme. Time-killing curve and post antifungal effects of Meifute antifungal solution against Candida albicans (ATCC90028) as the indicator fungal were measured.Result The MIC range, MIC₅₀ and MIC₉₀ of Meifute antifungal solution against 57 species were 1:40~1:160, 1:80 and 1:40 respectively. The ranges of the solution against Candida albicans and dermatophytes were 1:40~1:80 and 1:40~1:160. This antifungal solution has a strong antifungal effect. The degree and speed of the killing fungal were changed with the decrease of drug concentration. The PAFE of 0.5MIC, 1MIC and 2MIC were 0.85 h, 2.1 h and 3.59 h respectively, and the prolonged of PAFE was positively correlated to the antifungal solution concentration.Conclusion Meifute antifungal solution has a fast, sustained effect against Candida albicans and dermatophtes. Comparing with the effect on Candida albicans, the anigfungal solution have stronger antifungal effect on dermatophtes. The antifunal solution also have a long period of PAFE. These results showed that Meifute antifungal solution can be widely used to treat the superficial fungal infection of dermatophytes and Candida albicans.

Key words: minimal inhibitory concentration, time-kill curve, post antifungal effect

中图分类号:

R756.3

史冬梅, 顾克斌, 沈永年, 吕桂霞, 刘维达. 美浮特^®皮肤抗菌液对致足癣真菌抗真菌后效应的体外研究[J]. 中国真菌学杂志, 2014, 9(2): 88-92.

SHI Dong-mei, GU Ke-bing, SHEN Yong-nian, LÜ Gui-xia, LIU Wei-da. Post-antifungal effect of Meifute antifungal solution against fungal induced tinea pedis in vitro[J]. Chinese Journal of Mycology, 2014, 9(2): 88-92.

[1]	刘光荣, 翟春涛, 金敏蓉, 李成亮. 天然抗菌剂对5种致病菌的抑制作用研究[J]. 中国真菌学杂志, 2020, 15(2): 88-92.
[2]	郭惠仪, 周欣欣, 江丹. 4种中草药及其复方抗马拉色菌体的外药敏实验[J]. 中国真菌学杂志, 2013, 8(4): 210-213.
[3]	黄欣, 沈亮亮, 徐红, 温海. 8种中药单体抗球形马拉色菌的体外药敏实验[J]. 中国真菌学杂志, 2011, 6(2): 98-100.