医学真菌菌株保藏方法概述

中国真菌学杂志　 2014, Vol. 9 　Issue (2): 107-110.

医学真菌菌株保藏方法概述

桑军军^1,2, 邓淑文¹, 郭凯³, 潘炜华^1,2, 廖万清^1,2

1. 上海长征医院皮肤病与真菌病研究所全军真菌病重点实验室第二军医大学附属长征医院皮肤科, 上海 200003;
2. 上海市医学真菌分子生物学实验室, 上海 200003;
3. 第二军医大学附属长海医院血液科, 上海 200433

收稿日期:2013-12-17 出版日期:2014-04-28 发布日期:2014-04-28
通讯作者: 廖万清，liaowanqing@sohu.com；潘炜华，panweihua@medmail.com.cn E-mail:liaowanqing@sohu.com;panweihua@medmail.com.cn
作者简介:桑军军，男（汉族），博士研究生在读.E-mail：sangjunjunsang@163.com

Culture preservation of medical fungi

SANG Jun-jun^1,2, DENG Shu-wen¹, GUO Kai³, PAN Wei-hua^1,2, LIAO Wan-qing^1,2

1. Department of Dermatology, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China;
2. Shanghai Key Laboratory of Molecular Medical Mycology, Shanghai 200003, China;
3. Department of Hematology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China

Received:2013-12-17 Online:2014-04-28 Published:2014-04-28

摘要/Abstract

摘要：

近年来，随着真菌感染的患者急剧增加，真菌病已经成为一个重要的公共卫生问题。对医学真菌流行病学、致病机理、耐药机理以及防治策略的研究尤为重要，而所有研究的基础都建立在对医学真菌的进行有效的保藏。医学真菌的保藏方法很多，包括传代法、蒸馏水法、冷冻干燥保藏法等，保藏方法的选择因实验室条件、菌种和研究要求不同而不同。本文对目前常用的几种医学真菌菌种保藏方法的优缺点及其应用等做了综述。

关键词: 医学真菌, 菌种保藏, 冷冻保藏法, 冷冻干燥保藏法

Abstract:

In recent years, mycoses have emerged as an important public health problem. The research about fungal epidemiology,pathogenesis,drug-resistant mechanism, prevention and treatment of mycoses is particularly important. Culture collections are responsible for providing the microbial resources for development of biological sciences. There are many preservation techniques of medical fungi. The choice of the method will depend to a large extent on the equipment and facilities available in the laboratory. The paper discusses quality management options and reviews different methods of fungal preservation, describing how the reproducibility and quality of these techniques are maintained in order to retain the full potential of fungi.

Key words: medical fungi, culture collection, cryopreservation, freeze-drying

中图分类号:

R379

桑军军, 邓淑文, 郭凯, 潘炜华, 廖万清. 医学真菌菌株保藏方法概述[J]. 中国真菌学杂志, 2014, 9(2): 107-110.

SANG Jun-jun, DENG Shu-wen, GUO Kai, PAN Wei-hua, LIAO Wan-qing. Culture preservation of medical fungi[J]. Chinese Journal of Mycology, 2014, 9(2): 107-110.

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