CRK1基因缺失对白念珠菌形态、黏附、生物被膜的影响

中国真菌学杂志　 2014, Vol. 9 　Issue (1): 7-11.

CRK1基因缺失对白念珠菌形态、黏附、生物被膜的影响

赵静¹, 刘继勇², 张海³, 曹永兵⁴, 郑庆虎⁵, 吴建华¹

1. 上海长海医院皮肤科, 上海 200433;
2. 第二军医大学长海医院药材科, 上海 200433;
3. 第二军医大学肝胆医院药材科, 上海 200433;
4. 第二军医大学药学院药理学教研室, 上海 200433;
5. 解放军153中心医院皮肤科, 郑州 450042

收稿日期:2013-11-20 出版日期:2014-02-28 发布日期:2014-02-28
通讯作者: 郑庆虎，E-mail：zhengqinghu205@163.com；吴建华，E-mail：wujh_ch@163.com E-mail:zhengqinghu205@163.com;wujh_ch@163.com
作者简介:赵静，女（汉族），硕士研究生在读.E-mail：13807262695@163.com
基金资助:
国家973项目（2013CB531602）

The effect of CRK1 gene deletion on morphogenesis,adhesion and biofilm formation of Candida albicans

ZHAO Jing¹, LIU Ji-yong², ZHANG Hai³, CAO Yong-bing⁴, ZHEGN Qing-hu⁵, WU Jian-hua¹

1. Department of Dermatology, Changhai hospital, Shanghai 200433, China;
2. School of Pharmacy, Changhai hospital, Shanghai 200433, China;
3. School of Pharmacy, Eastern Hepatobiliary Surgery Hospital, Shanghai 200433, China;
4. School of Pharmacy, The Second Military Univercity, Shanghai 200433;
5.Department of Dermatology, No.153 Hospital of PLA, Zhengzhou 450042, China

Received:2013-11-20 Online:2014-02-28 Published:2014-02-28

摘要/Abstract

摘要： 目的研究CRK1基因缺失对白念珠菌形态、黏附、生物被膜的影响。方法显微镜下观察，计算菌丝形成率，比较CRK1基因缺失菌（Δcrk1菌）及标准菌SC5314形成菌丝的能力；建立肠黏膜模型，计算黏附率，评价CRK1基因缺失对白念珠菌黏附的影响；MTT法及结晶紫法（CV）评价CRK1基因缺失对白念珠菌生物被膜形成的影响。结果与SC5314相比，Δcrk1菌分别在10%胎牛血清和RPMI-1640培养条件下形成菌丝能力均较弱，两者之间有统计学差异；Δcrk1菌在60、90、120 min时对肠黏膜的黏附数明显少于标准菌SC5314，两者之间有统计学差异；通过MTT法、结晶紫法两种方法证实了，在经48 h培养后，Δcrk1菌与其标准菌SC5314相比，形成生物膜的能力弱，两者之间差异有统计学差异。结论 CRK1基因缺失影响白念珠菌菌丝二态性的转化，进而影响黏附力和生物被膜的形成。

关键词: 白念珠菌, CRK1基因, 形态, 黏附, 生物被膜

Abstract: Objective To observe if the CRK1 gene deletion influence its virulence of C.albicans.Methods Cells were observed microscopically for formation of hyphae. The numbers of adherent C.albicans were counted by colony counting method after interaction at different time. Biofilm was quantified by MTT assay and CV assay.Results Compared with SC5314, the Δcrk1 had a weak ability to form hyphae at the condition of 10% Serum and RPMI-1640 medium. The Δcrk1 had a significant reduction in adhension with human intestinal epithelial cells in vitro at 30,60,90,120 minutes. It was weak for the Δcrk1 to form biofilm quantified by CV-assay and MTT-assay.Conclusions CRK1 gene deletion can weaken its morphogenesis,adhesion and biofilm formation.

Key words: CRK1 gene deletion, Candida albicans, morphogenesis, adhesion, biofilm formation

中图分类号:

R379.4

赵静, 刘继勇, 张海, 曹永兵, 郑庆虎, 吴建华. CRK1基因缺失对白念珠菌形态、黏附、生物被膜的影响[J]. 中国真菌学杂志, 2014, 9(1): 7-11.

ZHAO Jing, LIU Ji-yong, ZHANG Hai, CAO Yong-bing, ZHEGN Qing-hu, WU Jian-hua. The effect of CRK1 gene deletion on morphogenesis,adhesion and biofilm formation of Candida albicans[J]. Chinese Journal of Mycology, 2014, 9(1): 7-11.

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