真菌多样性研究方法进展

摘要/Abstract

摘要： 真菌广泛存在于自然界，在生态系统中发挥着重要的作用。随着分子生物学技术在微生物多样性研究中的广泛应用以及宏基因组学技术的出现，打破了传统微生物培养法的局限性，人们对于真菌多样性的认识日渐提高。该文主要综述了研究真菌多样性方法的主要发展历程，介绍几种有关真菌多样性研究常用的分子生物学方法，包括基于PCR的克隆文库方法、变性梯度凝胶电泳、末端限制性长度多态性、实时荧光定量PCR、荧光原位杂交、序列标签标记的高通量测序以及宏基因组技术，并且阐述宏基因组学技术在此领域蕴含的巨大发展潜力。

关键词: 真菌, 多样性, 研究方法

Abstract: Fungi widely exists in nature, playing an important role in the ecosystem. The pervasive application of molecular biology technology in microbial diversity and the emergence of metagenomic breaked the limitations of traditional microbial culture method, so that improve the human's recognition of fungal diversity. This paper reviewed the main development course of studying fungal diversity approaches, introduced several commonly used methods of molecular biology on fungal diversity research, mainly including clone library PCR based, Denaturing gradient gel electrophoresis, Terminal restriction fragment length polymorphism, Real-time fluorescent quantitative PCR, Fluorescence in situ hybridization and barcoded pyrosequencing, and expounded the huge potential of metagenomics in this field.

Key words: fungi, diversity, method

中图分类号:

R379

石莉娜, 刘晓峰. 真菌多样性研究方法进展[J]. 中国真菌学杂志, 2014, 9(1): 60-64.

SHI Li-na, LIU Xiao-feng. Advances in the method of fungi diversity[J]. Chinese Journal of Mycology, 2014, 9(1): 60-64.

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