欢迎访问《中国真菌学杂志》杂志官方网站,今天是 分享到:

中国真菌学杂志 2014, Vol. 9  Issue (1): 28-30,35.

论著 上一篇    下一篇

297例浅部真菌病临床及病原菌菌种分析

张伟铮1, 陈力2, 赵瑾2, 陈江汉2   

  1. 1. 广东省中医院大学城医院检验科, 广州 510006;
    2. 上海长征医院皮肤病与真菌病研究所 全军真菌病重点实验室 第二军医大学附属长征医院皮肤科, 上海 200003
  • 收稿日期:2013-12-18 出版日期:2014-02-28 发布日期:2014-02-28
  • 通讯作者: 陈江汉,E-mail:chenjianghan@126.com E-mail:chenjianghan@126.com
  • 作者简介:张伟铮,男(汉族),硕士,主管技师.E-mail:zwzzw83@163.com

Analysis of 297 cases of superficial mycosis and its pathogenic fungi strains

ZHANG Wei-zheng1, CHEN Li2, ZHAO Jin2, CHEN Jiang-han2   

  1. 1. Department of Laboratory Medicine, The Tradition Chinese Hospital of Guangdong Province, Guangzhou 510006, China;
    2. Institute of dermatology and fungal disease, Key Laboratory of the army fungal disease, Department of dermatology, Changzheng Hospital, Shanghai 200003
  • Received:2013-12-18 Online:2014-02-28 Published:2014-02-28

摘要: 目的 探讨皮肤浅部真菌病致病真菌菌种的构成。方法 对297例真菌涂片阳性和培养阳性的浅部真菌病患者,取标本进行分离培养及菌种鉴定,培养阳性标本在形态学上不能准确鉴定的,进行梅里埃API 20C AUX酵母菌鉴定试剂盒或核糖体DNA(rDNA)ITS区序列测定,确切鉴定菌种。使用SPSS 17.0统计软件对于结果进行统计分析。结果 共分离培养出致病菌13种,其中红色毛癣菌86株(29.0%),须癣毛癣菌68株(22.9%),念珠菌属59株(19.9%),暗色真菌属13株(4.4%),曲霉菌属13株(4.4%),红酵母菌12株(4.0%),青霉菌属9株(3.0%),毛霉菌9株(3.0%),犬小孢子菌5株(1.7%),浅白隐球菌3株(1.0%),毛孢子菌属2株(0.7%),絮状表皮癣菌1株(0.3%),混合感染17株(5.7%)。结论 本地区浅部真菌病以甲癣为主,主要致病真菌是红色毛癣菌,但其他种类真菌感染尤其是念珠菌属有明显上升趋势。

关键词: 浅部真菌病, 真菌菌种, 鉴定

Abstract: Objective To study pathogenic fungi strains in 297 cases of superficial mycosis.Methods The clinical specimens from 297 patients with superficial mycosis, which presented positive results of the direct microscopic examination, were examined with the culture and identification of isolates of causative fungi. Sometimes accurate identification needed BioMerieux API 20C AUX or the sequences of ITS region of ribosomal DNA (rDNA) identify. The results were analysed by SPSS 17.0 statistical software.Results All 13 kinds of pathogenic fungi strains were isolated, including Trichophyton rubrum 86 strains (29.0%),Trichophyton mentagrophytes 68 strains (22.9%),Candida 59 strains (19.9%),Dematiaceous hyphomycetes 13 strains (4.4%),Aspergillus 13 strains (4.4%),Rhodotorula 12 strains (4.0%),Penicillium 9 strains (3.0%),Mucor 9 strains (3.0%),Microsporum canis 5 strains (1.7%),Cryptococcus albidus 3 strains (1.0%),Trichosporon 2 strains (0.7%),Epidermophyton floccosum 1 strains (0.3%) and 17 mixed fungal infection (5.7%).Conclusion The predominant superficial mycosis is onychomycosis, and the predominant pathogenic fungi strains is Trichophyton rubrum, but Candida infection have grown significantly.

Key words: superficial mycosis, fungi strains, identification

中图分类号: