MALDI-TOF MS技术在临床病原真菌鉴定中的应用进展

摘要/Abstract

摘要： 基质辅助激光解吸电离飞行时间质谱技术（MALDI-TOF-MS）目前是一种快速而可靠的微生物鉴定方法。随着可鉴定真菌谱的完善，MALDI-TOF MS 技术已逐步应用于临床常见致病酵母菌、酵母样真菌和丝状菌的鉴定中，本文将就此做一综述。

关键词: 基质辅助激光解吸电离飞行时间质谱, 真菌, 鉴定

Abstract: Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS)-based systems is a rapid and reliable method in identification of microorganism.With the improvement of identifiable fungal species spectrum,the application of MALDI-TOF MS systems have been introduced to identification of clinically pathogenic yeasts,yeast-like fungi,filamentous fungi and dermatophytes.

Key words: Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), identification

中图分类号:

R446.53

王冲, 占萍, 刘维达. MALDI-TOF MS技术在临床病原真菌鉴定中的应用进展[J]. 中国真菌学杂志, 2013, 8(6): 369-373.

WANG Chong, ZHAN Ping, LIU Wei-da. Advances in the application of Matrix-assisted laser desorption ionization time-of-flight mass spectrometry for identification of clinically pathogenic fungi[J]. Chinese Journal of Mycology, 2013, 8(6): 369-373.

参考文献

[1] Sendid B, Ducoroy P, Francois N, et al. Evaluation of MALDI-TOF mass spectrometry for the identification of medically-important yeasts in the clinical laboratories of Dijon and Lille hospitals[J]. Med Mycol, 2013, 51(1): 25-32.
[2] Seyfarth F, Wiegand C, Erhard M, et al. Identification of yeast isolated from dermatological patients by MALDI-TOF mass spectrometry[J]. Mycoses, 2012, 55(3): 276-280.
[3] Marklein G, Josten M, Klanke U, et al. Matrix-assisted laser desorption ionization-time of flight mass spectrometry for fast and reliable identification of clinical yeast isolates[J]. J Clin Microbiol, 2009, 47(9): 2912-2917.
[4] Stevenson LG, Drake SK, Shea YR, et al. Evaluation of matrix-assisted laser desorption ionization-time of flight mass spectrometry for identification of clinically important yeast species[J]. J Clin Microbiol, 2010, 48(10): 3482-3486.
[5] Bader O, Weig M, Taverne-Ghadwal L, et al. Improved clinical laboratory identification of human pathogenic yeasts by matrix-assisted laser desorption ionization time-of-flight mass spectrometry[J]. Clin Microbiol Infect, 2011, 17(9): 1359-1365.
[6] Dhiman N, Hall L, Wohlfiel SL, et al. Performance and cost analysis of matrix-assisted laser desorption ionization-time of flight mass spectrometry for routine identification of yeast[J]. J Clin Microbiol, 2011, 49(4): 1614-1616.
[7] Pinto A, Halliday C, Zahra M, et al. Matrix-assisted laser desorption ionization-time of flight mass spectrometry identification of yeasts is contingent on robust reference spectra[J]. PLOS ONE, 2011, 6(10): e25712.
[8] Quiles-Melero I, Garcia-Rodriguez J, Gomez-Lopez A, et al. Evaluation of matrix-assisted laser desorption/ionisation time-of-flight (MALDI-TOF) mass spectrometry for identification of Candida parapsilosis, C.orthopsilosis and C.metapsilosis[J]. Eur J Clin Microbiol Infect Dis. 2012, 31(1): 67-71.
[9] Hendrickx M, Goffinet JS, Swinne D, et al. Screening of strains of the Candida parapsilosis group of the BCCM/IHEM collection by MALDI-TOF MS[J]. Diagn Microbiol Infect Dis, 2011, 70(4): 544-548.
[10] Firacative C, Trilles L, Meyer W. MALDI-TOF MS enables the rapid identification of the major molecular types within the Cryptococcus neoformans/C.gattii species complex[J]. PLOS ONE, 2012, 7(5): e37566.
[11] McTaggart LR, Lei E, Richardson SE, et al. Rapid identification of Cryptococcus neoformans and Cryptococcus gattii by matrix-assisted laser desorption ionization-time of flight mass spectrometry[J]. J Clin Microbiol, 2011, 49(8): 3050-3053.
[12] Lavergne RA, Chauvin P, Valentin A, et al. An extraction method of positive blood cultures for direct identification of Candida species by Vitek MS matrix-assisted laser desorption ionization time of flight mass spectrometry[J]. Med Mycol, 2013, 51(6):652-656.
[13] Yaman G, Akyar I, Can S. Evaluation of the MALDI TOF-MS method for identification of Candida strains isolated from blood cultures[J]. Diagn Microbiol Infect Dis, 2012, 73(1): 65-67.
[14] Van Herendael BH, Bruynseels P, Bensaid M, et al. Validation of a modified algorithm for the identification of yeast isolates using matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF MS)[J]. Eur J Clin Microbiol Infect Dis, 2012, 31(5): 841-848.
[15] Cassagne C, Cella AL, Suchon P, et al. Evaluation of four pretreatment procedures for MALDI-TOF MS yeast identification in the routine clinical laboratory[J]. Med Mycol, 2013, 51(4):371-377.
[16] Goyer M, Lucchi G, Ducoroy P, et al. Optimization of the preanalytical steps of matrix-assisted laser desorption ionization-time of flight mass spectrometry identification provides a flexible and efficient tool for identification of clinical yeast isolates in medical laboratories[J]. J Clin Microbiol, 2012, 50(9): 3066-3068.
[17] Del CF, Masotti A, Onori M, et al. MALDI-TOF MS proteomic phenotyping of filamentous and other fungi from clinical origin[J]. J Proteomics, 2012, 75(11): 3314-3330.
[18] Alanio A, Beretti JL, Dauphin B, et al. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry for fast and accurate identification of clinically relevant Aspergillus species[J]. Clin Microbiol Infect, 2011, 17(5): 750-755.
[19] Iriart X, Lavergne RA, Fillaux J, et al. Routine identification of medical fungi by the new Vitek MS matrix-assisted laser desorption ionization-time of flight system with a new time-effective strategy[J]. J Clin Microbiol, 2012, 50(6): 2107-2110.
[20] Cassagne C, Ranque S, Normand AC, et al. Mould routine identification in the clinical laboratory by matrix-assisted laser desorption ionization time-of-flight mass spectrometry[J]. PLOS ONE, 2011, 6(12): e28425.
[21] Schrodl W, Heydel T, Schwartze VU, et al. Direct analysis and identification of pathogenic Lichtheimia species by matrix-assisted laser desorption ionization-time of flight analyzer-mediated mass spectrometry[J]. J Clin Microbiol, 2012, 50(2): 419-427.
[22] Coulibaly O, Marinach-Patrice C, Cassagne C, et al. Pseudallescheria/Scedosporium complex species identification by Matrix-Assisted Laser Desorption Ionization Time-Of-Flight Mass Spectrometry[J]. Med Mycol, 2011, 49(6): 621-626.
[23] Erhard M, Hipler UC, Burmester A, et al. Identification of dermatophyte species causing onychomycosis and tinea pedis by MALDI-TOF mass spectrometry[J]. Exp Dermatol, 2008, 17(4): 356-361.
[24] Nenoff P, Erhard M, Simon JC, et al. MALDI-TOF mass spectrometry -a rapid method for the identification of dermatophyte species[J]. Med Mycol, 2013, 51(1): 17-24.
[25] Alshawa K, Beretti JL, Lacroix C, et al. Successful identification of clinical dermatophyte and Neoscytalidium species by matrix-assisted laser desorption ionization-time of flight mass spectrometry[J]. J Clin Microbiol, 2012, 50(7): 2277-2281.
[26] Theel ES, Hall L, Mandrekar J, et al. Dermatophyte identification using matrix-assisted laser desorption ionization-time of flight mass spectrometry[J]. J Clin Microbiol, 2011, 49(12): 4067-4071.
[27] Keller BO, Sui J, Young AB, et al. Interferences and contaminants encountered in modern mass spectrometry[J]. Anal Chim Acta, 2008, 627(1): 71-81.
[28] Buskirk AD, Hettick JM, Chipinda I, et al. Fungal pigments inhibit the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis of darkly pigmented fungi[J]. Anal Biochem, 2011, 411(1): 122-128.