新生隐球菌通过Dectin-1上调单个核细胞miR-146a的表达

中国真菌学杂志　 2013, Vol. 8 　Issue (6): 325-328.

新生隐球菌通过Dectin-1上调单个核细胞miR-146a的表达

黄红宇, 刘晶波, 魏云玉, 严子禾, 韩志君

无锡市第二人民医院检验科, 无锡 214002

收稿日期:2013-09-26 出版日期:2013-12-28 发布日期:2013-12-28
通讯作者: 韩志君，E-mail：zjhan1125@163.com E-mail:zjhan1125@163.com
作者简介:黄红宇，女（汉族），硕士，主任技师.E-mail：hhy2174@hotmail.com
基金资助:
无锡市科技支撑计划（CES01N1111）

Up-regulation of miR-146a by Cryptococcus neoformans via Dectin-1

HUANG Hong-yu, LIU Jing-bo, WEI Yun-yu, YAN Zi-he, HAN Zhi-jun

Department of Clinical Laboratory, the Second People's Hospital of Wuxi, Wuxi 214002, China

Received:2013-09-26 Online:2013-12-28 Published:2013-12-28

摘要/Abstract

摘要： 目的研究miR-146a是否参与新生隐球菌感染免疫应答过程。方法采用RT-PCR检测了6例新生隐球菌性脑膜炎患者和6名健康个体外周血单个核细胞（PBMC）中miR-146a的表达。以热灭活新生隐球菌刺激来自健康个体的PBMC，并加入Dectin-1抑制剂昆布多糖，采用RT-PCR检测热灭活新生隐球菌和昆布多糖对PBMC中miR-146a表达的影响。结果新生隐球菌性脑膜炎患者PBMC中miR-146a的表达较健康个体明显增高。热灭活新生隐球菌可以上调PBMC中miR-146a的表达，昆布多糖可以削弱其上调miR-146a表达的能力。结论热灭活新生隐球菌可以通过Dectin-1受体上调miR-146a的表达。miR-146a参与了新生隐球菌感染免疫应答过程，值得进一步研究。

关键词: 新生隐球菌, Dectin-1, miR-146a

Abstract: Objective To investigate whether miR-146a involved in the immune response against Cryptococcus neoformans.Methods The relative expression of miR-146a in peripheral blood mononuclear cell of cryptococcal meningitis patients and healthy individuals was determined by RT-PCR. Heat-killed Cryptococcus and laminarin, a Dectin-1 blocker, were used to stimulate PBMCs from healthy individuals, miR-146a expression were analyzed.Results miR-146a was increased in the group of cryptococcal meningitis patients. Heat-killed Cryptococcus could increase miR-146a expression in PBMCs, and this effect could be partially inhibited by laminarin.Conclusions Cryptococcus up-regulate miR-146a expression via Dectin-1. Mir-146a was involved in the immune response against Cryptococcus and further study should be taken to identify its potential mechanisms.

Key words: Cryptococcus neoformans, Dectin-1, miR-146a

中图分类号:

R379.5

黄红宇, 刘晶波, 魏云玉, 严子禾, 韩志君. 新生隐球菌通过Dectin-1上调单个核细胞miR-146a的表达[J]. 中国真菌学杂志, 2013, 8(6): 325-328.

HUANG Hong-yu, LIU Jing-bo, WEI Yun-yu, YAN Zi-he, HAN Zhi-jun. Up-regulation of miR-146a by Cryptococcus neoformans via Dectin-1[J]. Chinese Journal of Mycology, 2013, 8(6): 325-328.

参考文献

[1] Akira S. Innate immunity to pathogens: Diversity in receptors for microbial recognition[J]. Immunol Rev, 2009, 227(1):5-8.
[2] Zhou Q, Murphy WJ. Immune response and immunotherapy to cryptococcus infections[J]. Immunol Res, 2006, 35(3):191-208.
[3] Akira S, Uematsu S, Takeuchi O. Pathogen recognition and innate immunity[J]. Cell, 2006, 124(4):783-801.
[4] Takeuchi O, Akira S. Pattern recognition receptors and inflammation[J]. Cell, 2010, 140(6):805-820.
[5] 朱清源, 戈宝学. microRNA与免疫调控[J]. 现代免疫学, 2011, 31(2): 3-6.
[6] Bartel DP. Micrornas: Target recognition and regulatory functions[J]. Cell, 2009, 136(2):215-233.
[7] Bushati N, Cohen SM. Microrna functions[J]. Annu Rev Cell Dev Biol, 2007, 23:175-205.
[8] Xiao C, Rajewsky K. Microrna control in the immune system: Basic principles[J]. Cell, 2009, 136(1):26-36.
[9] Taganov KD, Boldin MP, Chang KJ, et al. Nf-kappab-dependent induction of microrna mir-146, an inhibitor targeted to signaling proteins of innate immune responses[J]. Proc Natl Acad Sci U S A, 2006, 103 (33):12481-12486.
[10] Hou J, Wang P, Lin L, et al. Microrna-146a feedback inhibits rig-i-dependent type i ifn production in macrophages by targeting traf6, irak1, and irak2[J]. J Immunol, 2009, 183 (3):2150-2158.
[11] Nahid MA, Satoh M, Chan EK. Mechanistic role of microrna-146a in endotoxin-induced differential cross-regulation of tlr signaling[J]. J Immunol, 2011, 186 (3):1723-1734.
[12] Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative pcr and the 2(-delta delta c(t)) method[J]. Methods, 2001, 25 (4):402-408.
[13] Nahid MA, Pauley KM, Satoh M, et al. Mir-146a is critical for endotoxin-induced tolerance: Implication in innate immunity[J]. J Biol Chem, 2009, 284 (50):34590-34599.
[14] Androulidaki A, Iliopoulos D, Arranz A, et al. The kinase akt1 controls macrophage response to lipopolysaccharide by regulating micrornas[J]. Immunity, 2009, 31 (2):220-231.
[15] Sheedy FJ, Palsson-McDermott E, Hennessy EJ, et al. Negative regulation of tlr4 via targeting of the proinflammatory tumor suppressor pdcd4 by the microrna mir-21[J]. Nat Immunol, 2010, 11 (2):141-147.
[16] Williams AE, Perry MM, Moschos SA, et al. Role of mirna-146a in the regulation of the innate immune response and cancer[J]. Biochem Soc Trans, 2008, 36(Pt 6):1211-1215.
[17] Li L, Chen XP, Li YJ. Microrna-146a and human disease[J]. Scand J Immunol, 2010, 71 (4):227-231.
[18] 胡志德, 黄元兰, 孙懿, 等. 热灭活白念珠菌通过Dectin-1诱导外周血单个核细胞表达IL-12和IFN-γ[J]. 现代免疫学, 2011, 31(1): 48-51.