甲真菌病体外模型研究

中国真菌学杂志　 2013, Vol. 8 　Issue (5): 265-268.

甲真菌病体外模型研究

胡婵¹, 朱红梅¹, 谭宏月¹, 李平¹, 赵瑾¹, 陈力¹, 温海¹, 宋维芳²

1. 上海长征医院皮肤病与真菌病研究所全军真菌病重点实验室第二军医大学附属长征医院皮肤科, 上海 200003;
2. 厦门大学附属成功医院解放军第174医院, 厦门 361003

收稿日期:2013-09-21 出版日期:2013-10-28 发布日期:2013-10-28
通讯作者: 温海,E-mail:wenhai98@sohu.com;宋维芳,E-mail:xm.songweifang@163.com E-mail:wenhai98@sohu.com;xm.songweifang@163.com
作者简介:胡婵,女 (汉族),博士研究生在读.E-mail:hc729729@sina.com
基金资助:
解放军南京军区医学科技创新课题(09MA065)

The study of a simple in vitro onychomycosis model

HU Chan¹, ZHU Hong-mei¹, TAN Hong-yue¹, LI Ping¹, ZHAO Jin¹, CHEN Li¹, WEN Hai¹, SONG Wei-Fang²

1. Institute of dermatology and fungal disease, Key Laboratory of the army fungal disease, Department of dermatology, Changzheng Hospital, Shanghai 200003, China;
2. Department of Dermatology, Affiliated Chenggong Hospital of Xiamen University Xiamen No.174 Hospital, Xiamen 361003, China

Received:2013-09-21 Online:2013-10-28 Published:2013-10-28

摘要/Abstract

摘要： 目的构建一个简单、经济的新的甲真菌病体外模型。方法制备猪甲板,在其腹侧面中央黏贴一个“O”型圈,将受试菌液加入“O”型圈内,将甲板放置培养皿中培养,用大体观察及组织病理的方法动态观察不同真菌侵袭甲板的情况。结果用猪甲板制备甲真菌病体外模型,通过该模型观察了红色毛癣菌、须癣毛癣菌以及白念珠菌穿透甲板的能力。通过组织病理学方法观察到了真菌动态穿透甲板的过程。结论该模型适用于评估真菌对甲板的致病作用。

关键词: 甲真菌病, 体外模型, 皮肤癣菌, 白念珠菌

Abstract: Objective To determine a simple, novel and economic in vitro onychomycosis model.Method An innovatively designed "O" ring was applied to localize the fugal suspension on the ventral surface of the porcine hoof, then 50 μL of fungal suspension was applied. The hoof membranes were cultured in sterile petri dishes. The dynamic penetration of hoof plates was observed with gross appreance and histopathological examination.Result In this study we developed a novel in vitro model of onychomycosis with porcine hoof in which we observed the Trichophyton rubrum,Trichophyton mentagrophytes and Candida albicans dynamic permeation to the dorsal of porcine hoof both by gross appreance and histopathological examination and this model also enables one to evaluate how the fungi behave in the nail plate and changes in the localization.Conclusion The model facilitates monitoring of the nail pathogenicity.

Key words: onychomycosis, in vitro model, dermatophytes, Candida albicans

中图分类号:

R756.4

胡婵, 朱红梅, 谭宏月, 李平, 赵瑾, 陈力, 温海, 宋维芳. 甲真菌病体外模型研究[J]. 中国真菌学杂志, 2013, 8(5): 265-268.

HU Chan, ZHU Hong-mei, TAN Hong-yue, LI Ping, ZHAO Jin, CHEN Li, WEN Hai, SONG Wei-Fang. The study of a simple in vitro onychomycosis model[J]. Chinese Journal of Mycology, 2013, 8(5): 265-268.

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