新生隐球菌荚膜GXM对小鼠神经小胶质细胞产ATP能力及其凋亡的影响

中国真菌学杂志　 2013, Vol. 8 　Issue (5): 261-264.

新生隐球菌荚膜GXM对小鼠神经小胶质细胞产ATP能力及其凋亡的影响

周洁, 潘炜华, 廖万清

上海长征医院皮肤病与真菌病研究所全军真菌病重点实验室第二军医大学附属长征医院皮肤科, 上海 200003

收稿日期:2013-05-10 出版日期:2013-10-28 发布日期:2013-10-28
通讯作者: 廖万清,E-mail:liaowanqing@sohu.com E-mail:liaowanqing@sohu.com
作者简介:周洁,女 (汉族),博士研究生在读.E-mail:jjsunny_20081199@yahoo.cn

The effect of Cryptococcus neoformans capsular GXM on energy metabolism and apoptosis of mouse microglia

ZHOU Jie, PAN Wei-hua, LIAO Wan-qing

Institute of dermatology and fungal disease, Key Laboratory of the army fungal disease, Department of dermatology, Changzheng Hospital, Shanghai 200003

Received:2013-05-10 Online:2013-10-28 Published:2013-10-28

摘要/Abstract

摘要： 目的分析新生隐球菌荚膜多糖GXM对小鼠神经小胶质细胞能量代谢和凋亡的影响。方法 ①采用紫外分光光度计检测GXM干预后的神经小胶质细胞能量产物ATP含量的改变情况。②采用AnnexinV-异硫氰酸荧光素 (fluorescein isothiocyanate,FITC)/碘化丙啶 (propidium iodide,PI)双标记法流式细胞术检测GXM细胞诱导神经小胶质细胞凋亡的情况。结果 ① GXM体外可诱导神经小胶质细胞产ATP能力下降。② GXM体外可诱导神经小胶质细胞的凋亡。结论新生隐球菌可通过GXM诱导能量代谢紊乱和凋亡来对神经小胶质细胞产生影响。

关键词: 新生隐球菌, 荚膜多糖, GXM, 小胶质细胞, 凋亡

Abstract: Objective To detect the effect of Cryptococcus neoformans GXM on energy metabolism and apoptosis of mouse microglia cell.Method ①The capacity of ATP generation in each group was evaluated with UV spectrophotometric determination.②Annexin V-fluorescein isothiocyanate/propidium iodide double label Method was taken to detect apoptosis rate of BV-2 cells after co-incubation with GXM.Results ①The capacity of ATP generation decreased after intervention by GXM.②GXM was able to induce apoptosis in microglial cells in vitro.Conclusion Cryptococcus neoformans could exert effect on host cells via induction of energy metabolism disorder and apoptosis in microglia by GXM.

Key words: Cryptococcus neoformans, capsular polysaccharide, GXM, microglia, apoptosis

中图分类号:

R379.5

周洁, 潘炜华, 廖万清. 新生隐球菌荚膜GXM对小鼠神经小胶质细胞产ATP能力及其凋亡的影响[J]. 中国真菌学杂志, 2013, 8(5): 261-264.

ZHOU Jie, PAN Wei-hua, LIAO Wan-qing. The effect of Cryptococcus neoformans capsular GXM on energy metabolism and apoptosis of mouse microglia[J]. Chinese Journal of Mycology, 2013, 8(5): 261-264.

参考文献

[1] Zaragoza O, Rodrigues ML, De Jesus M, et al. The capsule of the fungal pathogen Cryptococcus neoformans[J]. Adv Appl Microbiol,2009,68:133-216.
[2] Buchanan KL, Murphy JW. What makes Cryptococcus neoformans a pathogen[J]? Emerg Infect Dis,1998,4(1):71-83.
[3] McClelland EE, Bernhardt P, Casadevall A. Estimating the relative contributions of virulence factors for pathogenic microbes[J]. Infect Immun, 2006,74(3):1500-1504.
[4] McFadden DC, De Jesus M, Casadevall A. The physical properties of the capsular polysaccharides from Cryptococcus neoformans suggest features for capsule construction[J]. J Biol Chem, 2006,281(4):1868-1875.
[5] Gates MA, Thorkildson P, Kozel TR. Molecular architecture of the Cryptococcus neoformans capsule[J]. Mol Microbiol,2004,52(1):13-24.
[6] O'Meara TR, Alspaugh JA. The Cryptococcus neoformans capsule: a sword and a shield[J]. Clin Microbiol Rev,2012,25(3):387-408.
[7] Grechi J, Marinho-Carvalho M, Zancan P,et al. Glucuronoxylomannan from Cryptococcus neoformans down-regulates the enzyme 6-phosphofructo-1-kinase of macrophages[J]. J Biol Chem,2011,286(17):14820-14829.
[8] Goldman DL, Casadevall A, Cho Y, et al. Cryptococcus neoformans meningitis in the rat[J]. Lab Invest,1996,75(6):759-770.
[9] Zhou Q, Gault RA, Kozel TR,et al. Protection from direct cerebral cryptococcus infection by interferon-gamma-dependent activation of microglial cells[J]. J Immunol, 2007, 178(9):5753-5761.
[10] Gupta AK. Types of onychomycosis[J]. Cutis,2001,68(2 Suppl):4-7.
[11] Wozniak KL, Levitz SM. Isolation and purification of antigenic components of Cryptococcus[J]. Methods Mol Biol,2009,470:71-83.
[12] Chiapello LS, Aoki MP, Rubinstein HR,et al. Apoptosis induction by glucuronoxylomannan of Cryptococcus neoformans[J]. Med Mycol, 2003,41(4):347-353.
[13] Kaur G, Han SJ, Yang I, et al. Microglia and central nervous system immunity[J]. Neurosurg Clin N Am, 2010,21(1):43-51.
[14] Retini C, Vecchiarelli A, Monari C, et al. Encapsulation of Cryptococcus neoformans with glucuronoxylomannan inhibits the antigen-presenting capacity of monocytes[J]. Infect Immun,1998,66(2):664-669.
[15] Vecchiarelli A, Pietrella D, Lupo P,et al. The polysaccharide capsule of Cryptococcus neoformans interferes with human dendritic cell maturation and activation[J]. J Leukoc Biol,2003,74(3):370-378.
[16] Cross CE, Bancroft GJ. Ingestion of acapsular Cryptococcus neoformans occurs via mannose and beta-glucan receptors, resulting in cytokine production and increased phagocytosis of the encapsulated form[J]. Infect Immun,1995,63(7):2604-2611.
[17] Zaragoza O, Chrisman CJ, Castelli MV, et al. Capsule enlargement in Cryptococcus neoformans confers resistance to oxidative stress suggesting a mechanism for intracellular survival[J]. Cell Microbiol,2008,10(10):2043-2057.
[18] Syme RM, Bruno TF, Kozel TR,et al. The capsule of Cryptococcus neoformans reduces T-lymphocyte proliferation by reducing phagocytosis, which can be restored with anticapsular antibody[J]. Infect Immun, 1999,67(9):4620-4627.
[19] Dong ZM, Murphy JW. Intravascular cryptococcal culture filtrate (CneF) and its major component, glucuronoxylomannan, are potent inhibitors of leukocyte accumulation[J]. Infect Immun,1995,63(3):770-778.
[20] Vecchiarelli A, Retini C, Pietrella D, et al. Downregulation by cryptococcal polysaccharide of tumor necrosis factor alpha and interleukin-1 beta secretion from human monocytes[J]. Infect Immun,1995,63(8):2919-2923.
[21] Monari C, Paganelli F, Bistoni F, et al. Capsular polysaccharide induction of apoptosis by intrinsic and extrinsic mechanisms[J]. Cell Microbiol,2008,10(10):2129-2137.
[22] Monari C, Pericolini E, Bistoni G, et al. Cryptococcus neoformans capsular glucuronoxylomannan induces expression of fas ligand in macrophages[J]. J Immunol,2005,174(6):3461-3468.
[23] Villena SN, Pinheiro RO, Pinheiro CS, et al. Capsular polysaccharides galactoxylomannan and glucuronoxylomannan from Cryptococcus neoformans induce macrophage apoptosis mediated by Fas ligand[J]. Cell Microbiol,2008,10(6):1274-1285.
[24] Aubert M, Jerome KR. Apoptosis prevention as a mechanism of immune evasion[J]. Int Rev Immunol,2003,22(5-6):361-371.