无绿藻对小鼠Th17细胞分化的影响

摘要/Abstract

摘要： 目的研究无绿藻是否能够影响小鼠Th17细胞的分化,影响Th17细胞分泌相关的细胞因子。方法体外培养无绿藻,用尼龙毛柱法分离培养小鼠脾脏T淋巴细胞,按照1∶5的比例将两者在transwell培养板上共培养,培养2 h、4 h、8 h、12 h、24 h、48 h,分别收集T淋巴细胞,留取培养上清液。流式细胞技术检测Th17细胞表型,ELISA法检测培养上清液中IL-17的水平。结果从共培养2 h开始,随着共培养时间的延长Th17细胞所占比例逐渐降低,共培养8 h后趋于稳定 (P<0.05);从共培养2 h开始,培养上清液中IL-17的水平逐渐升高,8 h后出现下降,24 h后趋于稳定 (P<0.05)。结论在与T细胞共培养的条件下,无绿藻抑制了小鼠Th17细胞的分化,但是在最初2~8 h可以促进Th17相关细胞因子IL-17的释放。

关键词: 无绿藻, Th17, 流式细胞术, 细胞因子

Abstract: Objective To study if prototheca could influence the differentiation of mice Th17 cells and the secretion of Th17 associated cytokines.Methods Prototheca was cultured in vitro, and T lymphocytes were isolated from spleen of mice by nylon wool column, and cultured in vitro. Prototheca and T cells were co-cultured in transwell plate with a ratio of 1:5. Cells were culture for 2 h,4 h,8 h,12 h,24 h,48 h,and then T cells and supernatant were collected respectively, and the ratio of Th17 cell was analyzed with flow cytometry, and the level of IL-17 in the supernatant was detected with ELISA.Results From 2 h on, with the prolonged incubation time, the proportion of Th17 cells decreased gradually, and it became stable 8 h later (P<0.05); the level of IL-17 in the supernatant increased at earlier 2-8 h, and decreased from 8 h on, and it became stable 24 h later (P<0.05).Conclusion Being co-cultured with T cells, Prototheca could inhibit the differentiation of mice Th17 cells, but it could promote the secretion of IL-17 at the earlier 2-8 h.

Key words: prototheca, Th17, flow cytometry, cytokine

中图分类号:

R379.9

康俞莉, 赵颖, 章强强. 无绿藻对小鼠Th17细胞分化的影响[J]. 中国真菌学杂志, 2013, 8(5): 257-260.

KANG Yu-li, ZHAO Ying, ZHANG Qiang-qiang. The effect of prototheca on the differentiation of mice Th17 cells[J]. Chinese Journal of Mycology, 2013, 8(5): 257-260.

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