球形孢子丝菌致婴儿固定型孢子丝菌病1例及相关实验研究

中国真菌学杂志　 2013, Vol. 8 　Issue (4): 228-231.

球形孢子丝菌致婴儿固定型孢子丝菌病1例及相关实验研究

王润超, 王爱平, 万喆, 李若瑜

北京大学第一医院皮肤性病科北京大学真菌和真菌病研究中心, 北京 100034

收稿日期:2013-07-29 出版日期:2013-08-28 发布日期:2013-08-28
通讯作者: 王爱平,E-mail:wangap@medmail.com.cn E-mail:wangap@medmail.com.cn
作者简介:王润超,男(汉族),博士研究生在读.E-mail:wrczzl@163.com

A case of infant fixed sporotrichosis caused by Sporothrix globosa and laboratory study

WANG Run-chao, WANG Ai-ping, WAN Zhe, LI Ruo-yu

Department of Dermatology and Venereology, Peking University First Hospital, Peking University Research Center for Medical Mycology, Beijing 100034, China

Received:2013-07-29 Online:2013-08-28 Published:2013-08-28

摘要/Abstract

摘要： 报道1例由球形孢子丝菌所致的婴儿固定型孢子丝菌病。患儿女,3个月,因左眼下内侧皮损2个月就诊,皮损脓液标本进行真菌培养,对培养获得菌株进行形态学、生理学和分子生物学鉴定,并进行药物敏感性检测。真菌培养阳性,镜下可见典型的套袖样菌丝。钙调蛋白基因序列分析鉴定为球形孢子丝菌。药敏试验显示特比萘芬和伊曲康唑对该菌株的菌丝相最低抑菌浓度 (minimal inhibitory concentration,MIC)分别为0.5 μg/mL和0.5 μg/mL;对该菌株的酵母相的MIC值分别为0.25 μg/mL和0.5 μg/mL。给予患者口服特比萘芬32.5 mg/d治疗10周后皮损消退呈瘢痕化修复。依据临床及实验室检查确诊该病例为球形孢子丝菌所致固定型孢子丝菌病,特比萘芬治疗本病例显示较好疗效。

关键词: 婴儿, 孢子丝菌病, 球形孢子丝菌, 特比萘芬

Abstract: A case of infant fixed sporotrichosis caused by Sporothrix globosa was reported.A 3-month-old infant was admitted with skin granulomatous lesions beside the left medial canthus for 2 months.Direct smear examination and fungal culture, carbon source assimilation, PCR and calmodulin sequence analysis were performed for diagnosis.The antifungals susceptibility was tested according to M27-A3 and M38-A2 published by CLSI. Flower-shaped conidia and sleeve-like hyphae were observed in the slide culture. Calmodulin sequence were amplified,and the product was sequenced,which was aligned with 100% sequential similarity to multiple sequences of Sporothrix globosa in the GenBank database.The MICs for terbinafine and itraconazole were 0.5 μg/mL and 0.5 μg/mL against hyphae phage of the strain and 0.25 μg/mL and 0.5 μg/mL against yeast phage. The lesion was cured after oral terbinafine for 10 weeks. The case of fixed sporotrichosis was caused by Sporothrix globosa, terbinafine has good effect.

Key words: infant, sporotrichosis, Sporothrix globosa, terbinafine

中图分类号:

R756.6

王润超, 王爱平, 万喆, 李若瑜. 球形孢子丝菌致婴儿固定型孢子丝菌病1例及相关实验研究[J]. 中国真菌学杂志, 2013, 8(4): 228-231.

WANG Run-chao, WANG Ai-ping, WAN Zhe, LI Ruo-yu. A case of infant fixed sporotrichosis caused by Sporothrix globosa and laboratory study[J]. Chinese Journal of Mycology, 2013, 8(4): 228-231.

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