伊曲康唑联合卡泊芬净或特比萘芬对球形孢子丝菌体外抑菌作用的研究

中国真菌学杂志　 2013, Vol. 8 　Issue (1): 20-25.

伊曲康唑联合卡泊芬净或特比萘芬对球形孢子丝菌体外抑菌作用的研究

谭静文, 刘伟, 刘伟霞, 张晋卿, 孙毅, 万喆, 李若瑜

北京大学第一医院皮肤性病科, 北京大学真菌和真菌病研究中心, 皮肤病分子诊断北京市重点实验室, 北京 100034

收稿日期:2012-10-26 出版日期:2013-02-28 发布日期:2013-02-28
通讯作者: 刘伟,E-mail:liuwei@bjmu.edu.cn E-mail:liuwei@bjmu.edu.cn
作者简介:谭静文,女(汉族),硕士研究生在读.E-mail:cecilia88903@bjmu.edu.cn
基金资助:
教育部新世纪优秀人才支持计划(NCET-10-0198);国家自然科学基金重点项目(30930006);国家自然科学基金(30970131);北京市自然科学基金(7102149)

In vitro interactions of itraconazole with caspofungin or terbinafine against Sporothrix globosa

TAN Jing-wen, LIU Wei, LIU Wei-xia, ZHANG Jin-qing, SUN Yi, WAN Zhe, LI Ruo-yu

Department of Dermatology, Peking University First Hospital, Research Center for Medical Mycology, Beijing Key Laboratory of Molecular Diagnosis on Dermatoses, Peking University, Beijing 100034, China

Received:2012-10-26 Online:2013-02-28 Published:2013-02-28

摘要/Abstract

摘要： 目的探讨伊曲康唑联合卡泊芬净或特比萘芬时对球形孢子丝菌酵母相和菌丝相的体外抑菌作用。方法参照标准的微量液基稀释法及棋盘微量稀释法对33株球形孢子丝菌行药敏试验,结果使用抑菌浓度指数(FICI)判定"协同""不相关"和"拮抗"。结果伊曲康唑与卡泊芬净联合对球形孢子丝菌酵母相菌株的协同率为93.94%(31/33),与特比萘芬联合的协同率为60.61%(20/33);伊曲康唑与卡泊芬净或特比萘芬联合对菌丝相菌株协同率分别为96.97%(32/33)、84.5%(28/33)。对所有受试菌株均未观察到上述药物的拮抗作用。结论体外联合药敏试验结果显示伊曲康唑与卡泊芬净或特比萘芬联合有较好的协同作用。

关键词: 球形孢子丝菌, 菌丝相, 酵母相, 卡泊芬净, 伊曲康唑, 特比萘芬, 联合药敏

Abstract: Sporotrichosis is a common fungal infection with limited therapeutic strategies.Combination of synergistic drugs could provide new angle for treating this disease. We used the standard broth microdilution and checkerboard method to evaluate the in vitro efficacy of combining itraconazole with caspofungin or terbinafine against the yeast and mycelial phases of Sporothrix globosa. Fractional inhibitory concentration index (FICI) was used to interprete the result as following: synergistic if the FICI is ≤0.5; indifferent if the FICI is >0.5 but ≤4 and antagonistic if the FICI is >4. For yeast phase, the synergic interaction of itraconazole combined with caspofungin or terbinafine was 93.94% (31/33) and 60.61% (20/33), respectively; for mycelial phase, the synergic interaction of itraconazole combined with caspofungin or terbinafine was 96.97% (32/33) and 84.5% (20/33), respectively. Antagonism was not observed. The in vitro efficacy of combining itraconazole with caspofungin or terbinafine showed perfect synergy against S.globosa, suggesting a potential therapeutic choice for treatment of Sporotrichosis.

Key words: Sporothrix globosa, mycelial form, yeast form, caspofungin, itraconazole, terbinafine, combined drug susceptibility testing

中图分类号:

R978.5

谭静文, 刘伟, 刘伟霞, 张晋卿, 孙毅, 万喆, 李若瑜. 伊曲康唑联合卡泊芬净或特比萘芬对球形孢子丝菌体外抑菌作用的研究[J]. 中国真菌学杂志, 2013, 8(1): 20-25.

TAN Jing-wen, LIU Wei, LIU Wei-xia, ZHANG Jin-qing, SUN Yi, WAN Zhe, LI Ruo-yu. In vitro interactions of itraconazole with caspofungin or terbinafine against Sporothrix globosa[J]. Chinese Journal of Mycology, 2013, 8(1): 20-25.

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