关键词: 万博节皮菌(趾间毛癣菌), 非培养法, 药敏试验, 转化医学真菌学

Abstract: Objective To report a case of facial tinea incognito due to Arthroderma vanbreuseghemii,identified by direct DNA extraction from the scales,and compared with that from the isolated colony.The isolated colony was tested in vitro for drug susceptibility.Methods The scales scraped from the affected area were prepared for PCR and fungal culture.DNA was extracted directly from the scale and amplified using the universal fungal primers ITS1/4,the sequences of amplification products were blasted in GenBank.After the pathogen was isolated,the drug susceptibility test was performed using the commercialized anti-fungal creams.Results The case was finally diagnosed as facial tinea incognito due to A.vanbreuseghemii,the teleomorph of Trichophyton interdigitale.The patient was cured after 5 weeks oral terbinafine(250 mg/d) and topical application of antifungal cream containing 1%naftifine-0.25%ketoconazole after 5 weeks.Conclusions PCR-based molecular biological validation of DNA from scales with the ability of rapid identification for pathogenic fungus and using anti-fungal cream directly for in vitro susceptibility test could be a good guidance for direct treatment and monitor during the therapy.This individual diagnosis and treatment program is a successful model of translational medical mycology from bedside to bench and from bench to bedside.

Key words: Arthroderma vanbreuseghemii, culture-independent PCR, in vitro susceptibility test, translational medical mycology