卡泊芬净、米卡芬净对念珠菌体外药物敏感性的动态研究

中国真菌学杂志　 2011, Vol. 6 　Issue (5): 276-280.

卡泊芬净、米卡芬净对念珠菌体外药物敏感性的动态研究

曾荣¹, 李岷¹, 陈青², 王乐¹, 吕桂霞¹, 沈永年¹, 蔡晴¹, 李彩霞¹, 唐荣才², 刘维达¹

1. 中国医学科学院北京协和医学院皮肤病研究所, 南京210042;
2. 江苏省血液中心, 南京210042

收稿日期:2011-07-15 出版日期:2011-10-28 发布日期:2011-10-28
通讯作者: 李岷,E-mail:drlimin@sina.cn;陈青,E-mail:qngchen@hotmail.com E-mail:drlimin@sina.cn;qngchen@hotmail.com
作者简介:曾荣,男(汉族),硕上研究生在读.E-mail:zengrong2010@hotmail.com
基金资助:
中国医学科学院皮肤病研究所重点项目基金(PYSZ200601)

Dynamic study on the susceptibilities of caspofungin and micafungin to Candida species in vitro

ZENG Rong¹, LI Min¹, CHEN Qing², WANG Le¹, LV Gui-xia¹, SHEN Yong-nian¹, CAI qing¹, LI Cai-xia¹, TANG Rong-cai², LIU Wei-da¹

1. Institute of Dermatology, Chinese Academy of Medical Science & Peking Union Medical College, Nanjing 210042;
2. Jiangsu Province Blood Center, Nanjing 210042

Received:2011-07-15 Online:2011-10-28 Published:2011-10-28

摘要/Abstract

摘要： 目的动态研究卡泊芬净、米卡芬净体外对念珠菌的药物敏感性。方法参照CLSI公布的M-27A方案微量液体稀释法分别测定卡泊芬净、米卡芬净、氟康唑对85株念珠菌的体外敏感性,并连续7 d观测结果。结果 48 h卡泊芬净对白念珠菌、光滑念珠菌及其他念珠菌MIC₅₀、MIC₉₀中位数分别为0.030μg/mL、0.030μg/mL,0.060μg/mL、0.125μg/mL,0.125μg/mL、0.500μg/mL。48 h米卡芬净对白念珠菌、光滑念珠菌及其他念珠菌MIC₅₀、MIC₉₀中位数分别为0.030μg/mL、0.030μg/mL,0.060μg/mL、0.060μg/mL,0.250μg/mL、0.500μg/mL。48 h氟康唑对白念珠菌、光滑念珠菌及其他念珠菌MIC₈₀、MIC₁₀₀中位数分别为2μg/mL、128μg/mL,64μg/mL、128μg/mL,2μg/mL、32μg/mL。85株念珠菌中未见对3种药物同时耐药的菌株。卡泊芬净组白念珠菌MIC₅₀、MIC₉₀ 24 h后不再升高;光滑念珠菌MIC₅₀ 72 h后不再升高,MIC₉₀ 120 h后不再升高;其他念珠菌组MIC₅₀ 168 h、MIC₉₀ 96 h后不再升高。米卡芬净组白念珠菌、光滑念珠菌MIC₅₀、MIC₉₀ 24 h后不再升高;其他念珠菌MIC₅₀、MIC₉₀在72 h后不再升高。结论卡泊芬净、米卡芬净对念珠菌属有较好的抗菌作用,其中对白念珠菌、光滑念珠菌作用更强,且MICs随着作用时间延长而升高并存在药物特异性和念珠菌种属特异性。

关键词: 动态, 卡泊芬净, 米卡芬净, 氟康唑, 念珠菌, 体外药敏

Abstract: Objective To assess the susceptibilities of Candida species to caspofungin and micafungin.Methods Susceptibilities of Candida isolates to caspofungin,micafungin and fluconazole were determined by microdilution method based on CLSI M27-A2 for consecutive seven days.Results After 48 hours,the medians of MIC₅₀ and MIC₉₀ of C.albicans,C.glabrata,and other Candida spp.to caspofungin were 0.03 and 0.03 μg/mL,0.06 and 0.125 μg/mL,0.125 and 0.5 μg/mL,respectively.The medians of MIC₅₀ and MIC₉₀ of C.albicans,C.glabrata,and other Candida species to micafungin were 0.03 μg/mL and 0.03 μg/mL,0.06 μg/mL and 0.06 μg/mL,0.25 μg/mL and 0.5 μg/mL,respectively.The medians of MIC₈₀ and MIC₁₀₀ of C.albicans,C.glabrata,and other Candida species to fluconazole were 2 μg/mL and 128 μg/mL,64 μg/mL and 128 μg/mL,2 μg/mL and 32 μg/mL,respectively.None of the 85 isolates of Candida species showed cross-resistance to caspofungin,micafungin or fluconazole.To caspofungin,there was no change on MIC₅₀ or MIC₉₀ of C.albicans after 24 hours.No increase on MIC₅₀ and MIC₉₀ of C.glabrata was observed after 72 hours and 120 hours.Neither did increase on MIC₅₀ and MIC₉₀ of other Candida species after 168 hours and 96 hours.To micafungin,there was no change on MIC₅₀ and MIC₉₀ of C.albicans and C.glabrata after 24 hours and no increase on MIC₅₀ and MIC₉₀ of other Candida species after 72 hours.Conclusions Caspofungin and micafungin may have great antifungal activities to all candida species,especially to C.albicans and C.glabrata.The MICs would increase with the exposure time,which show specificity to drug and Candida species.

Key words: dynamic, caspofungin, micafungin, fluconazole, Candida species, in vitro susceptibility

中图分类号:

R379.4

曾荣, 李岷, 陈青, 王乐, 吕桂霞, 沈永年, 蔡晴, 李彩霞, 唐荣才, 刘维达. 卡泊芬净、米卡芬净对念珠菌体外药物敏感性的动态研究[J]. 中国真菌学杂志, 2011, 6(5): 276-280.

ZENG Rong, LI Min, CHEN Qing, WANG Le, LV Gui-xia, SHEN Yong-nian, CAI qing, LI Cai-xia, TANG Rong-cai, LIU Wei-da. Dynamic study on the susceptibilities of caspofungin and micafungin to Candida species in vitro[J]. Chinese Journal of Mycology, 2011, 6(5): 276-280.

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