2种中药单体对念珠菌的体外药敏研究

中国真菌学杂志　 2011, Vol. 6 　Issue (1): 26-30.

2种中药单体对念珠菌的体外药敏研究

姜淼, 黄欣, 沈亮亮, 周斐, 屠韡燕, 施伟民

同济大学附属同济医院皮肤科, 上海, 200065

收稿日期:2010-12-30 出版日期:2011-02-28 发布日期:2011-02-28
通讯作者: 黄欣,E-mail:alida_huang@yahoo.com.cn E-mail:alida_huang@yahoo.com.cn
作者简介:姜淼,女(汉族),硕士,主治医师.E-mail:jiang_miao@126.com
基金资助:
上海市卫生局青年科研基金(2006Y53)

Antifungal sensitivity of two Chinese traditional drugs against Candida in vitro

JIANG Miao, HUANG Xin, SHEN Liang-liang, ZHOU Fei, TU Wei-yan, SHI Wei-min

Department of Dermatology, Tongji Hospital, Tongji University School of Medicine, Shanghai 200065

Received:2010-12-30 Online:2011-02-28 Published:2011-02-28

摘要/Abstract

摘要： 目的探讨人参茎叶皂甙、三七总皂甙、氟康唑、伊曲康唑体外抗念珠菌的敏感性。方法采用CLSI公布的M-27A方案微量液体稀释法分别测定氟康唑、伊曲康唑、人参茎叶皂甙、三七总皂甙对60株临床分离念珠菌的体外敏感性。结果氟康唑、伊曲康唑、人参茎叶皂甙、三七总皂甙对受试菌的MIC₅₀分别为0.25、0.25、7.812和15.625 mg/L,MIC₉₀分别为64、16、250和250 mg/L。60株念珠菌临床分离株中有10株对伊曲康唑、人参茎叶皂甙、三七总皂甙交叉耐药。结论人参茎叶皂甙、三七总皂甙在体外对念珠菌均有不同程度抑制作用,CLSI M27-A方案可用于对中药抗念珠菌活性的评价。

关键词: 念珠菌, 微生物敏感试验, 氟康唑, 伊曲康唑, 人参茎叶皂甙, 三七总皂甙

Abstract: Objective To compare the susceptibilities of Candida albicans cultured with ginseng stems and leaves saponins,Panax Pseudoginseng,fluconazole and itraconazole.Methods Susceptibilities of 60 candida isolates cultured with fluconazole,itraconazole,ginseng stems and leaves saponins and Panax Pseudoginseng was determined by M27-A Microdilution Method based on CLSI.Results The MIC₅₀ and MIC₉₀ of Candida albicans to fluconazole,itraconazole,ginseng stems and leaves saponins and Panax Pseudoginseng were 0.25 mg/L,0.25 mg/L,7.812 mg/L,15.625 mg/L(MIC₅₀),and 64 mg/L,16 mg/L,250 mg/L,250 mg/L(MIC₉₀),respectively.Ten of the total 60 isolates showed cross-resistances to itraconazole,ginseng stems and leaves saponins and Panax Pseudoginseng.Conclusions Ginseng stems and leaves saponins and Panax Pseudoginseng have similar antifungal effects on Candida albicans.CLSI-M27-A could be used to evaluate the anti-candida activity to the components of medicinal plant as a standard method.

Key words: Candida, microbial susceptibility tests, fluconazole, itraconazole, ginseng stems and leaves saponins, Panax Pseudoginseng

中图分类号:

R379.4

姜淼, 黄欣, 沈亮亮, 周斐, 屠韡燕, 施伟民. 2种中药单体对念珠菌的体外药敏研究[J]. 中国真菌学杂志, 2011, 6(1): 26-30.

JIANG Miao, HUANG Xin, SHEN Liang-liang, ZHOU Fei, TU Wei-yan, SHI Wei-min. Antifungal sensitivity of two Chinese traditional drugs against Candida in vitro[J]. Chinese Journal of Mycology, 2011, 6(1): 26-30.

参考文献

[1] Ono F,Yasumoto S.Genital candidiasis[J].Nippon Rinsho,2009,67(1):157-161.
[2] pappas PG,Kauffman CA,Andes D.Clinical practice guidelines for the management of candidiasis:2009 update by the infectious diseases society of America[J].Clin Infect Dis,2009,48(5):503-535.
[3] National Committee for Clinical laboratory Standards.Reference method for broth dilution antifungal susceptibility testing of yeasts[S].Approved standard M27-A.1997.
[4] 宫毓静,安汝国,虞慧,等.164种中药乙醇提取物抗真菌作用研究[J].中草药,2002,33(1):42-47.
[5] 闻平,陈蕾,俞晓丽,等.胡桃揪提取物抗白念珠菌作用的机制研究[J].中药药理与临床,2008,24(1):51-53.
[6] Seneviratne CJ,Wong RW,Samaranayake LP.Potent anti-microbial activity of traditional Chinese medicine herbs against Candida species[J].Mycoses,2008,51(1):30-34.
[7] Keith I.Immune system effects of echinacea,ginseng,and astragalus:a review[J].Integrative Cancer Therapies,2003,2(3):247-267.
[8] Chapagain,Wiesman.In vitro study of the antifungal activity of saponin-rich extracts against prevalent phytopathogenic fungi[J].Industrial crops and products,2007,26(2):109-115.
[9] Sojakova M,Liiyrajoya D,Messer SA,et al.Fluconazole and itraconazole susceptibility of vaginal yeast isolates from Slovakia[J].Mycopathologia,2004,157(2):163-169.
[10] Perea S,Patterson TF.Antifungal resistance in pathogenic fungi[J].Clin Infect Dis,2002,35(9):1073-1080.
[11] Muller FM,Staudigel A,Salvenmoser S,et al.Cross-resistalce to medical and agricultural azole drugs in yeasts from the oropharynx of human immunodeficiency virus patients and from environmental Bavarian vine grapes[J].Antimicrob Agents Chemother,2007,57(4):3014-3016
[12] Sabatellis F,Patel R,Sobel JD,et al.In vitro activities of posaconzole,fluconazole,itraconazole,voriconazole and amphotericin B against a large collection of clinically important molds and yeasts[J].Antimicrob Agents Chemother,2006,50(6):2009-015.

[1]	杨靖娴, 邵冬华, 郭莉娜, 刘静, 徐英春, 梁国威. 北京某医院侵袭性念珠菌感染的菌株分布及药敏分析[J]. 中国真菌学杂志, 2020, 15(6): 359-363,370.
[2]	郭艳阳, 孙笑然, 海璐明, 闫东, 朱冠男, 马翠玲, 王刚, 付萌. 人工性脂膜炎伴发近平滑念珠菌感染1例分析[J]. 中国真菌学杂志, 2020, 15(6): 368-370.
[3]	黄晶晶, 范欣, 肖盟, 徐志鹏, 张戈, 徐英春. 培养条件对克柔念珠菌MALDI-TOF MS鉴定结果的影响分析[J]. 中国真菌学杂志, 2020, 15(5): 262-267.
[4]	黄进宝, 兰长青, 翁恒, 林清华. 肋骨隐球菌感染2例报道并文献复习[J]. 中国真菌学杂志, 2020, 15(5): 278-282.
[5]	刘丹, 邢丽枝, 杨心茹, 罗伟, 何荣霞. 唑类药物治疗外阴阴道假丝酵母菌病研究进展[J]. 中国真菌学杂志, 2020, 15(5): 318-320.
[6]	张文, 罗莹, 国承杰, 徐英春, 肖盟, 类承斌, 赵燕, 毕蕾. 院内念珠菌血症临床特点及病原菌分析[J]. 中国真菌学杂志, 2020, 15(3): 154-160.
[7]	李晓君, 谭俊青, 潘慧娟, 王康椿. 老年患者导尿管相关念珠菌泌尿系感染菌株分布及药敏分析[J]. 中国真菌学杂志, 2020, 15(3): 161-163.
[8]	马玉帛, 张逸, 黄晨, 王晓雯, 李若瑜. 自然杀伤细胞在真菌感染免疫的研究进展[J]. 中国真菌学杂志, 2020, 15(3): 175-178.
[9]	邱熙然, 陈思敏, 侯炜彤, 张玉, 郭诗雨, 姜远英, 安毛毛. 白念珠菌侵袭宿主毒力因子研究进展[J]. 中国真菌学杂志, 2020, 15(3): 183-188.
[10]	张静云, 彭靖雯, 王琼, 高盈, 陈万鑫, 沈永年, Li Dong-mei, 佘晓东, 刘维达. 表皮生长因子受体在阴道念珠菌病发病过程中的作用初探[J]. 中国真菌学杂志, 2020, 15(2): 65-71.
[11]	赵德军, 杨淋. 贵州省某区域女性阴道念珠菌的菌种分布及耐药性分析[J]. 中国真菌学杂志, 2020, 15(2): 83-87.
[12]	刘光荣, 翟春涛, 金敏蓉, 李成亮. 天然抗菌剂对5种致病菌的抑制作用研究[J]. 中国真菌学杂志, 2020, 15(2): 88-92.
[13]	谭静文, 徐红, 宋金凤, 高志琴, 杨虹, 杨连娟, 温海. 聚六亚甲基双胍体外抗真菌作用研究[J]. 中国真菌学杂志, 2020, 15(2): 93-96.
[14]	阚思玥, 蔡晴, 金云, 陈伟, 胡素泉, 杨连娟, 刘维达. 婴幼儿皮肤黏膜念珠菌病临床特点及其病原菌体外药敏分析[J]. 中国真菌学杂志, 2020, 15(2): 97-100.
[15]	霍晓赛, 贺政新, 王缚鲲. 侵袭性念珠菌感染及其与定植的关系研究进展[J]. 中国真菌学杂志, 2020, 15(2): 125-128.