红色毛癣菌菌株的特异性PCR鉴定方法研究

中国真菌学杂志　 2010, Vol. 5 　Issue (5): 282-285.

红色毛癣菌菌株的特异性PCR鉴定方法研究

费实, 高爱莉, 黎小东, 李振洁, 肖常青, 朱慧兰

广州市皮肤病防治所, 广州, 510095

收稿日期:2010-07-03 出版日期:2010-10-28 发布日期:2010-10-28
通讯作者: 朱慧兰,E-mail:zhlhuilan@hotmail.com E-mail:zhlhuilan@hotmail.com
作者简介:费实,女(汉族),本科,副主任技师.E-mail:alicegao197897@163.com

PCR identification of Trichophyton rubrum

FEI Shi, GAO Ai-li, LI Xiao-dong, LI Zhen-jie, XIAO Chang-qing, ZHU Hui-lan

Guang zhou Institute of Dermatology and Venerology, Guang zhou 510095, China

Received:2010-07-03 Online:2010-10-28 Published:2010-10-28

摘要/Abstract

摘要： 目的建立一种快速的红色毛癣菌分子生物学鉴定方法。方法根据红色毛癣菌保守区域-真菌核糖体DNA(rDNA)的转录间隔区(ITS)设计特异性引物,采用上游:ITS19865’GAC ACC AAG AAA AAA TTC TCT GAA GA3’,下游:ITS24415’GTC CTG AGG GCG CTG AA3’为引物对45株红色毛癣菌、5株须癣毛癣菌和1株紫色毛癣菌菌株的DNA进行PCR扩增,观察产物电泳带型的差异。结果 45株红色毛癣菌均能扩增出目的片段,5株须癣毛癣菌和1株紫色毛癣菌均无目的片段扩增出。结论红色毛癣菌可用特异引物PCR方法快速鉴定。

关键词: 红色毛癣菌, PCR, 须癣毛癣菌, 皮肤癣菌

Abstract: Obiective To develop a rapid method to for identification of Trichophyton rubrum.Methods Primed ITS1986 5’GAC ACC AAG AAA AAA TTC TCT GAA GA3’,ITS2441 5’GTC CTG AGG GCG CTG AA3’ were chosen to amplify 45 strains of Trichophyton rubrum,5 strains of Trichophyton mentagrophytes and 1 strain of Trichophyton violaceum.Results Characteristic bands were amplified in Trichophyton rubrum.But there was no characteristic band found in Trichophyton mentagrophytes and Trichophyton violaceum.Conclusions Trichophyton rubrum could be identificated by PCR rapidly.

Key words: Trichophyton rubrum, PCR, Trichophyton mentagrophytes, dermatophytes

中图分类号:

R379.2

费实, 高爱莉, 黎小东, 李振洁, 肖常青, 朱慧兰. 红色毛癣菌菌株的特异性PCR鉴定方法研究[J]. 中国真菌学杂志, 2010, 5(5): 282-285.

FEI Shi, GAO Ai-li, LI Xiao-dong, LI Zhen-jie, XIAO Chang-qing, ZHU Hui-lan. PCR identification of Trichophyton rubrum[J]. Chinese Journal of Mycology, 2010, 5(5): 282-285.

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