白念珠菌SSK1突变株对氟康唑敏感性的初步研究

中国真菌学杂志　 2010, Vol. 5 　Issue (5): 269-272.

白念珠菌SSK1突变株对氟康唑敏感性的初步研究

李筱芳¹, Neeraj Chauhan², Richard Calderone³, 刘维达¹

1. 中国医学科学院、北京协和医科大学皮肤病研究所, 南京, 210042;
2. Public Health Research Institute Center, New Jersey Medical School, Newark, New Jersey 07103;
3. Georgetown University Medical Center, Washington, DC 20057

收稿日期:2010-04-28 出版日期:2010-10-28 发布日期:2010-10-28
通讯作者: 刘维达,E-mail:liumyco@hotmail.com E-mail:liumyco@hotmail.com
作者简介:李筱芳,女(白族),博士,主治医师.E-mail:lxf3568@gmail.com
基金资助:
中央级公益性科研院所基本科研业务专项基金(YZ-07-3)

Antifungal activity of fluconazole against Ssk1p mutant of Candida albicans

LI Xiao-fang¹, Neeraj Chauhan², Richard Calderone³, LIU Wei-da¹

1. Institute of Dermatology, Chinese Academy of Medical Sciences & Peking Union Medical College, Nanjing 210042;
2. Public Health Research Institute Center, New Jersey Medical School, Newark, New Jersey 07103;
3. Georgetown University Medical Center, Washington, DC 20057

Received:2010-04-28 Online:2010-10-28 Published:2010-10-28

摘要/Abstract

摘要： 目的探讨氟康唑作用于白念珠菌双组分信号传导途径SSK1突变株SSK21后药物敏感性的变化。方法采用微量液体稀释法和固醇测定法测定野生株(CAF2-1)和突变株(SSK21)的最小抑菌浓度(MIC);并应用RT-PCR观察氟康唑作用前后,SSK1的表达变化。结果氟康唑对CAF2-1的MIC为16μg/mL,对SSK21为0.032μg/mL。加入氟康唑后,CAF2-1的SSK1表达明显增加,60min时达到最多。结论 SSK21对氟康唑高度敏感,SSK1基因及其相关的重要基因与药物敏感性的关系值得进一步研究,从而为新的抗真菌药物和治疗途径的研发提供参考。

关键词: 白念珠菌, 双组分信号传导途径, 氟康唑, 药物敏感性

Abstract: Objective To investigate the in vitro antifungal activity of fluconazole against two-component signal transduction protein Ssk1p mutant of Candida albicans (SSK21).Methods The minimum inhibitory concentrations (MIC) of fluconazole against SSK21 and wild type of C.albicans (CAF2-1) were tested by broth microdilution and ergosterol content assays.RT-PCR was performed to determine the expression of SSK1 in CAF2-1 in the presence of fluconazole.Results The MIC of fluconazole against SSK21 was 0.032 μg/mL,while that against CAF2-1 was 16 μg/mL.After administration with fluconazole,the transcription level of SSK1 from CAF2-1 was increased.Conclusions SSK21 is hypersensitive to fluconazole compared with wild type strain CAF2-1.Ssk1p and related genes should be exploited for new therapeutic approach.

Key words: Candida albicans, two-component signal transduction, fluconazole, drug sensitivity

中图分类号:

R379.4

李筱芳, Neeraj Chauhan, Richard Calderone, 刘维达. 白念珠菌SSK1突变株对氟康唑敏感性的初步研究[J]. 中国真菌学杂志, 2010, 5(5): 269-272.

LI Xiao-fang, Neeraj Chauhan, Richard Calderone, LIU Wei-da. Antifungal activity of fluconazole against Ssk1p mutant of Candida albicans[J]. Chinese Journal of Mycology, 2010, 5(5): 269-272.

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